Sirna Library Screening

Genome wide sirna and microrna libraries.

Sirna library screening.

Fast and easy online configuration and ordering of plated sirna microrna reagents. Rnai screening has allowed researchers to study the functionality of genes in a cell both individually and in. A breast cancer cell line was transfected with a sirna library that targeted a variety of specific kinases. The number of patients with cell types selleck screening library determined according to world health organization criteria were 2 5 7 37 and 7 for types a ab b1 b2.

Sirna library provides a new and efficient way that bring about many original breakthroughs in biology especially for the discovery of new targets combined with. The sirna library brings a revolution in the efficiency of functional genomics research. In the pursuit of gene functionality rnai library screening has become a crucial research tool used to study gene function and comprehensive signaling pathways. Rna interference rnai is a conserved biological response to the presence of double stranded rna which mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids and regulates the expression of protein coding genes.

Take advantage of predefined gene families including druggable genes or whole genome. Prepare sirna liposome complexes in the wells of 96 well plates. Sirna library screening protocol. Screening often lies at the beginning of most discovery research.

It enables high throughput low cost ultra fast gene function screening disease mechanism research target discovery drug development etc. Library screening using genome wide sirnas as well as micrornas allows a researcher to analyze an entire signaling pathway of one or tens of thousands of genes. Sirna library minimize time cost of screening using highly functional sirna. The silencer select human apoptosis sirna library provides investigators with a focused sirna screening platform targeting the most up to date apoptosis gene targets thus enabling a detailed analysis of the apoptotic signaling pathway the silencer select human apoptosis sirna library contains three unique non overlapping sirnas per target for 924 genes.

Learn more and view all available silencer sirna libraries. 1 transfection of cells with sirna pools is performed using reverse transfection. Skip to primary content. Cancer research 2012 14 r22.

Adapted from hu et al. Sirna libraries composed of large numbers of sirnas have been developed and are used to identify sets of genes functionally involved in a particular biological process or disease. To better understand this complexity researchers are moving toward large screening projects to identify candidate genes involved in their biological question of interest.